February 2013 Self-Test: AMP Case Report Article in CAP TODAY

Multilocus sequencing for rapid identification of molds

  1. Zygomycoses are important to recognize and treat aggressively in immunocompromised patients due to a highly morbid and often fatal clinical course. Which statement is false?
    1. Zygomycosis may cause a rapidly fatal, clinically obscure infection even in non-immunocompromised patients.
    2. As a class of antifungals, azoles have good efficacy against Zygomycetes.
    3. The stomach is the most common site of infection in gastrointestinal zygomycoses.
    4. Zygomycoses may infect a wide range of tissue sites, possibly leading to uncommon presentations that evade early detection.
  2. In tissue, zygomycosis may show variation from normal characteristic morphology (variable-width ribbon-like hyphae, irregular branching, aseptate), but are typically PAS positive and silver negative. Classic Zygomycete morphology includes:
    1. 45-degree branching.
    2. Narrow filamentous hyphae.
    3. Budding yeast forms.
    4. Fruiting bodies.
    5. No to weak reactivity with silver special stains.
  3. Molecular identification of organisms in paraffin-embedded tissues is a rapid and accurate diagnostic test, and if done in a timely manner, can guide clinical therapies. Which diagnostic modality cannot identify fungal organisms beyond the genera level?
    1. Fungal culture.
    2. Ribosomal DNA sequencing.
    3. Histomorphology and special stains.
    4. MALDI-TOF mass spectrometry.

ANSWERS to Self-Test for February 2013 Case

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