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Association for Molecular Pathology

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For AMP Webinars that offer Continuing Education Credit:


AMP is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program and by the Florida Board of Clinical Laboratory Personnel.


Individual attendees requesting P.A.C.E.® credit should use the form provided below. If you are viewing the webinar in a group setting, please use the P.A.C.E.® Group Attendance Roster, below. Each group participant is required to print and sign his or her name and email address to verify attendance. Once the webinar has concluded, the designated "Site Coordinator" must email this group attendance form to Kathleen Carmody, P.A.C.E.® Coordinator, in order for each participant to receive P.A.C.E.® credit.

 

Please contact Kathleen Carmody via email with any questions.


Next-Generation Sequencing (NGS) Technologies for HIV Drug Resistance Testing

Thursday, June 18, 2015

1:00pm Eastern

 

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PDF Handout

 

P.A.C.E.® Group Attendance Roster


P.A.C.E.® Individual Evaluation Form

Certificate of Attendance

Presenter: Robert W. Shafer, MD
Stanford University School of Medicine

Stanford, CA


Host: Benjamin Pinsky, MD

Stanford University School of Medicine

Stanford, CA


Description:

HIV-1 exists within individuals as a complex mixture of variants often referred to as a quasispecies. During the past 10 years, there have been many studies of the distribution and clinical significance of "low-abundance" or "minority" drug-resistance variants present in plasma in proportions below 20% to 30% -- the limit of detection of standard genotypic resistance testing using dideoxy-terminator "Sanger" sequencing. HIV-1 "deep" sequencing using NGS technologies have been widely used in research settings to study the clinical significance of low-abundance drug resistance mutations. In addition, NGS is commercially available for several niche clinical applications such as determining HIV-1 tropism before using a CCR5 inhibitor.
In this webinar, Dr. Shafer will review the scenarios in which low-abundance HIV-1 drug-resistance mutations are most clinically relevant, the most influential studies of NGS for HIV-1 drug resistance testing, and the practical aspects of NGS that have so far slowed its widespread adoption for most routine HIV-1 genotypic resistance testing.

 

Learning Objectives:

1. Describe the prevalence and clinical significance of low-abundance HIV-1 drug resistance testing in newly infected antiretroviral naive patients and in patients with a history of antiretroviral treatment failure.

2. Explain the promises and limitations of NGS deep sequencing for HIV-1 drug resistance testing including the published experience with the 454, Ion Torrent, PacBio, and Illumina sequencing platforms.

3. Recognize the practical laboratory and bioinformatic considerations that must be understood before implementing NGS deep sequencing for research or clinical applications

 

Level of Instruction: Basic

 

Number of Contact Hours: 1.0

 

AMP is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program and by the Florida Board of Clinical Laboratory Personnel.

 

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Wednesday, July 1, 2015

12:00pm Eastern

Development, Optimization and Implementation of Genomic Analysis Pipelines Using Next Generation Sequencing Technologies

 

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PDF Handout

 

P.A.C.E.® Group Attendance Roster


P.A.C.E.® Individual Evaluation Form

Certificate of Attendance

 

 

 

 

 

Presenter: Raghu Metpally, PhD

Weis Center for Research, Geisinger Clinic

Danville, PA


Host: N. Sertac Kip, MD, PhD

Geisinger Health System

Danville, PA

More information coming soon!

 

 

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Practical Applications of Next-Generation Sequencing in Clinical Microbiology

Tuesday, August 11, 2015

1:00pm Eastern

 

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PDF Handout

 

P.A.C.E.® Group Attendance Roster


P.A.C.E.® Individual Evaluation Form

Certificate of Attendance

Presenter: Stephen J. Salipante, MD, PhD

University of Washington

Seattle, WA


Host: Benjamin Pinsky, MD

Stanford University School of Medicine

Stanford, CA

 

Description:

This webinar will be a discussion about current and future applications of next-generation sequencing technologies in clinical microbiology laboratories. A special focus will be placed on assays that are currently validated for clinical use.

 

Learning Objectives:

1) Recognize the major sequencing technologies currently used in clinical laboratories.

2) Describe the current applications of next-generation sequencing data in clinical laboratories.

3) Identify future opportunities for integration of next-generation sequencing technologies into clinical laboratories.

Level of Instruction:  Intermediate

 

Number of Contact Hours: 1.0

 

AMP is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program and by the Florida Board of Clinical Laboratory Personnel.

 

Register Now

 



NGS 101 Presentation #3: An Integrated Approach to Assay Selection and Validation

To be Rescheduled

 

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PACE Group Attendance Roster


PACE Individual Evaluation Form

Certificate of Attendance

 

Handout

 

 

 

 

 

 

 

 

 

Presenter: Avni B. Santani, PhD

The Children's Hospital of Philadelphia

Philadelphia, PA

 

Host: Matthew Hiemenz, MD

University of Pennsylvania Health System
Philadelphia, PA

 

Description:

This webinar will provide an overview of factors important in clinical NGS test development and validation guidelines for clinical NGS tests. The professional standards and guidelines for quality and compliance that have been developed to date will be discussed and aspects such as instrument validation, assay/test validation, quality management, proficiency testing, and the use of reference materials during routine testing will be emphasized.

 

Learning Objectives:

1. Recognize the factors important in clinical NGS test development.
2. Review guidelines for optimizing and validating clinical NGS tests and platforms.
3. Prepare to implement a quality management program to ensure accurate NGS test results.

 

Level of Instruction: Basic